Dongdong Li
  • E-mail :[email]
  • Phone : +33 1 44 27 33 57
  • Location : Paris, France
Last update 2021-04-21 11:32:35.228

Dongdong Li PhD in Biomedical Engineering

Course and current status

2010 -               Researcher at INSERM (National Institute of Health and Medical Research/L'Institut national de la santé et de la recherche médicale, France), Paris

2005 - 2010      Post-doc, Neurophysiology and new microscopies laboratory, INSERM, Université Paris Descartes, Paris

2000 - 2005      PhD Biomedical engineering, Huazhong univ sci&tech, Wuhan

1993 - 1997      B.E. Electrical automation, Hebei univ tech, Tianjin

Scientific summary

Biophysical investigation of neuron&glia signaling

The mammalian nervous system consists of comparable number of neurons and glial cells. An important challenge now in neuroscience is to understand how neurons and glial cells co-operate to regulate the function and health of the nervous system.

We currently study the cellular mechanisms of neuron-glia signaling, ranging from second messenger dynamics (e.g., Ca2+, cAMP), transmitter release, to vesicle/receptor trafficking. Not only will it provide integrative knowledge on neurophysiology, but also help to identify dysregulations in neuron/glia actions in diseases. A multidisciplinary approach is being employed, including (but not limited to) quantitative imaging, pharmaco-/opto-genetics, and electrophysiology.

 

Publications (https://orcid.org/0000-0002-6731-4771):

eLife. 2021 April;10:e68128. doi: 10.7554/eLife.68128. Liu Q, Wang J, Wei X, Hu J, Ping C, Gao Y, Xie C, Wang P, Cao P, Cao Z, Yu Y, Li D, Yao J. Therapeutic inhibition of keratinocyte TRPV3 sensory channel by local anesthetic dyclonine. 

Acta Neuropathol Commun. 2021 March 9: 44. https://doi.org/10.1186/s40478-021-01146-1. Pham C, Hérault K, Oheim M, Maldera S, Vialou V, Cauli B, Li DAstrocytes respond to a neurotoxic Ab fragment with state-dependent Ca2+ alteration and multiphasic transmitter release. 

Front Cell Dev Biol. 2021 Feb 9;9:634160. doi: 10.3389/fcell.2021.634160. Hu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, Zhang Y, Xie C, Li D, Yao J. Flotillin-1 interacts with and sustains the surface levels of TRPV2 channel.  

J Biol Chem. 2020 october. doi: 10.1074/jbc.RA120.015605. Wang Y, Mo X, Ping C, Huang Q, Zhang H, Xie C, Zhong B, D Li, J Yao. Site-specific contacts enable distinct modes of TRPV1 regulation by the potassium channel Kvb1 subunit. 

NeuroImage. 2020 June, https://doi.org/10.1016/j.neuroimage.2020.117069. Pham C, Herrera Moro D, Mouffle C, Didienne S, Hepp R, Pfrieger FW, Mangin JM, Legendre P, Martin C, Luquet S, Cauli B, Li D. Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening. 

Proc Natl Acad Sci U S A. 2019 Feb 25; Tian Q, Hu J, Xie C, Mei K, Pham C, Mo X, Hepp R, Soares S, Nothias F, Wang Y, Liu Q, Cai F, Zhong B, Li D, Yao J. Recovery from tachyphylaxis of TRPV1 coincides with recycling to the surface membrane. doi: 10.1073/pnas.1819635116. 

J NeurosciAstorga G, Li D, Therreau L, Kassa M, Marty A&Llano I. Concerted interneuron activity in the cerebellar molecular layer during rhythmic oromotor behaviors.     

Neuroscience. 2016 May 26;323:76-83. Ropert N, Jalil A, Li D. Expression and cellular function of vSNARE proteins in brain astrocytes. 

J Physiol. 2015 Jul 1;593(13):2807-32. (cover). Li D, Hérault K, Zylbersztejn K, Lauterbach MA, Guillon M, Oheim M, Ropert N. Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking. 

Front Cell Neurosci. 2013 Oct 29;7:193. Li D, Agulhon C, Schmidt E, Oheim M, Ropert N. New tools for investigating astrocyte-to-neuron communication.  (review invited)

J Neurosci. 2013 Mar 6;33(10):4434-55. Li D, Hérault K, Silm K, Evrard A, Wojcik S, Oheim M, Herzog E, Ropert N.Lack of evidence for vesicular glutamate transporter expression in mouse astrocytes.

J Am Chem Soc. 2012 Sep 12;134(36):14923-31. Collot M, Loukou C, Yakovlev AV, Wilms CD, Li D, et al.  Calcium rubies: a family of red-emitting functionalizable indicators suitable for two-photon Ca2+ imaging. 

J Physiol. 2012 Feb 15;590(4):855-73. Li D, Hérault K, Isacoff EY, Oheim M, Ropert N. Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release. 

Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21960-5. Li D, Hérault K, Oheim M, Ropert N. FM dyes enter via a store-operated calcium channel and modify calcium signaling of cultured astrocytes. 

Cell Calcium 2008. 45:275-283. Luccardini C, Yakovlev A, Pasche M, Gaillard S, Li D, et al. Measuring mitochondrial and cytoplasmic Ca2+ in EGFP expressing cells with a low-affinity CALCIUM RUBY and its dextran conjugate. 

J Neurosci. 2008 Jul 23;28(30):7648-58. Li D, Ropert N, Koulakoff A, Giaume C, Oheim M. Lysosomes are the major vesicular compartment undergoing Ca2+-regulated exocytosis from astrocytes.  

Biophys J. 2007 Aug 1;93(3):969-80. Nadrigny F, Li D, Kemnitz K, Ropert N, Koulakoff A, Rudolph S, Vitali M, Giaume C, Kirchhoff F, Oheim M. Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles. 

In Imaging Cellular and Molecular Biological Function. - F. Frischknecht, J. Pawley and S. Shorte, eds. Springer, Berlin Heidelberg. 2006. pp.117–156. Oheim M and Li D. Quantitative Co-localisation Imaging: Concepts, Measurements and Pitfalls. 

Traffic 2006 7:416-428. Bai L, Zhu D, Zhou K, Zhou W, Li D, et al. Differential properties of GTP- and Ca2+-stimulated exocytosis from large dense core vesicles. 

Acta Biophysica Sinica 2005 21:261-268. Liang T, Li D, Xiong J, Zhu D, Qu A. Dynamic difference between GLUT4 storage vesicles and secretory vesicles revealed by evanescent-field imaging and single particle tracking. 

Proc Natl Acad Sci U S A 2005 102:123-127. Liu D, Xu L, Yang F, Li D, Gong F, and Xu T. Rapid biogenesis and sensitization of secretory lysosomes in NK cells mediated by target-cell recognition. 

Neural Interface and Control –IEEE 2005pp.225-229. Li D, Liang T, and Qu A. Quantitative analysis of diffraction-limited three-dimensional single secretory vesicle tracking in live neuroendocrine cells. 

Conf Proc IEEE Eng Med Biol Soc 2005. 7:7529–7532. Xiong J, Li D, Zhu D, and Qu A. Three-Dimensional tracking of single granules in living PC-12 cells employing TIRFM and WFFM. 

Biophys J 2004 87:1991-2001. Li D, Xiong J, Qu A, and Xu T. Three-dimensional tracking of single secretory granules in live PC12 cells.

Cell Res 2004 14:480-486. Li C, Bai L, Li D, Xia S, and Xu T. Dynamic tracking and mobility analysis of single GLUT4 storage vesicle in live 3T3-L1 cells. 

Acta Biochim et Biophys Sin 2003 35:671-676. Li D, Guo X, Qu A, and Xu T. Use of three-dimensional fluorescence deconvolution microscopy for study of spatial distribution of secretory vesicles in living cells. 

Acta Biophys Sin 2003 19:286-290. Xiong J, Li D, Qu A, and Xu T. Studies on the method of visualization of three-dimensional images of living cells. 

Image d’exemple