2015 - INSERM Researcher CR1/HDR, from Mars/2016, Neuroscience Paris Seine, University Paris 6 - UPMC. (Jan/2015 - Feb/2016, Brain physiology lab, University Paris 5 - Paris Descartes)
2010 - 2014 INSERM Researcher CR2, Neurophysiology and new microscopies lab, University Paris 5 - Paris Descartes
2005 - 2010 Post-doc, Neurophysiology and new microscopies laboratory, INSERM (National institute of health and medical research, France)
2000 - 2005 PhD Biomedical engineering, Huazhong univ sci&tech, Wuhan, China
1993 - 1997 B.E. Electrical automation, Hebei univ tech, Tianjin, China
Neuron, Glia & Their Interaciton
The mammalian central nervous system (CNS) consists of comparable number of neurons and glial cells. An important challenge now in neuroscience is to understand how neurons and glial cells co-operate to regulate CNS function and health.
My study aims at unraveling the mechanisms of neuron-glia interactions. Not only will it provide integrative knowledge on neurophysiology, but also help to identify dysregulations in neuroglial interactions in diseases. A multidisciplinary approach is being employed for in situ investigation, including (but not limited to) quantitative imaging, pharmaco-/opto-genetics, and electrophysiology.
Ropert N, Jalil A, Li D. Expression and cellular function of vSNARE proteins in brain astrocytes. Neuroscience. 2016 May 26;323:76-83.
Li D, Hérault K, Zylbersztejn K, Lauterbach MA, Guillon M, Oheim M, Ropert N. Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking. J Physiol. 2015 Jul 1;593(13):2807-32.
Li D, Agulhon C, Schmidt E, Oheim M, Ropert N. New tools for investigating astrocyte-to-neuron communication. Front Cell Neurosci. 2013 Oct 29;7:193.
Li D, Hérault K, Silm K, Evrard A, Wojcik S, Oheim M, Herzog E, Ropert N. Lack of evidence for vesicular glutamate transporter expression in mouse astrocytes. J Neurosci. 2013 Mar 6;33(10):4434-55. doi: 10.1523/JNEUROSCI.3667-12.2013.
Collot M, Loukou C, Yakovlev AV, Wilms CD, Li D, et al. Calcium rubies: a family of red-emitting functionalizable indicators suitable for two-photon Ca2+ imaging. J Am Chem Soc. 2012 Sep 12;134(36):14923-31.
Li D, Hérault K, Isacoff EY, Oheim M, Ropert N. Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release. J Physiol. 2012 Feb 15;590(4):855-73.
Li D, Hérault K, Oheim M, Ropert N. FM dyes enter via a store-operated calcium channel and modify calcium signaling of cultured astrocytes. Proc Natl Acad Sci U S A. 2009 Dec 22;106(51):21960-5.
Luccardini C, Yakovlev A, Pasche M, Gaillard S, Li D, et al. Measuring mitochondrial and cytoplasmic Ca2+ in EGFP expressing cells with a low-affinity CALCIUM RUBY and its dextran conjugate. Cell Calcium 2008. 45:275-283
Li D, Ropert N, Koulakoff A, Giaume C, Oheim M. Lysosomes are the major vesicular compartment undergoing Ca2+-regulated exocytosis from cortical astrocytes. J Neurosci. 2008 Jul 23;28(30):7648-58. doi: 10.1523/JNEUROSCI.0744-08.2008.
Nadrigny F, Li D, Kemnitz K, Ropert N, Koulakoff A, Rudolph S, Vitali M, Giaume C, Kirchhoff F, Oheim M. Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles. Biophys J. 2007 Aug 1;93(3):969-80.
Oheim M and Li D. Quantitative Co-localisation Imaging: Concepts, Measurements and Pitfalls. In Imaging Cellular and Molecular Biological Function. - F. Frischknecht, J. Pawley and S. Shorte, eds. Springer, Berlin Heidelberg.
Bai L, Zhu D, Zhou K, Zhou W, Li D, et al. Differential properties of GTP- and Ca2+-stimulated exocytosis from large dense core vesicles. Traffic 2006 7:416-428.
Liang T, Li D, Xiong J, Zhu D, Qu A. Dynamic difference between GLUT4 storage vesicles and secretory vesicles revealed by evanescent-field imaging and single particle tracking. Acta Biophysica Sinica 2005 21:261-268.
Liu D, Xu L, Yang F, Li D, Gong F, and Xu T. Rapid biogenesis and sensitization of secretory lysosomes in NK cells mediated by target-cell recognition. Proc Natl Acad Sci U S A 2005 102:123-127.
Li D, Liang T, and Qu A. Quantitative analysis of diffraction-limited three-dimensional single secretory vesicle tracking in live neuroendocrine cells. Neural Interface and Control –IEEE 2005. pp.225-229.
Xiong J, Li D, Zhu D, and Qu A. Three-Dimensional tracking of single granules in living PC-12 cells employing TIRFM and WFFM. Conf Proc IEEE Eng Med Biol Soc 2005. 7:7529–7532.
Li D, Xiong J, Qu A, and Xu T. Three-dimensional tracking of single secretory granules in live PC12 cells. Biophys J 2004 87:1991-2001.
Li C, Bai L, Li D, Xia S, and Xu T. Dynamic tracking and mobility analysis of single GLUT4 storage vesicle in live 3T3-L1 cells. Cell Res 2004 14:480-486.
Li D, Guo X, Qu A, and Xu T. Use of three-dimensional fluorescence deconvolution microscopy for study of spatial distribution of secretory vesicles in living cells. Acta Biochim et Biophys Sin 2003 35:671-676.
Xiong J, Li D, Qu A, and Xu T. Studies on the method of visualization of three-dimensional images of living cells. Acta Biophs Sin 2003 19:286-290.