• E-mail :[email]
  • Phone : +33 4 93953425
  • Location : Valbonne, France
Last update 2011-08-23 22:49:03.79

Massimo MANTEGAZZA PhD Physiology

Course and current status

Current Position

Inserm CR1 researcher - ATIP/Avenir Group Leader, Institut de Pharmacologie Moléculaire et Cellulaire (IPMC), CNRS UMR6097 and University of Nice-Sophia Antipolis, 660 Route des Lucioles, 06560 Valbonne, France Tel. 04 93953425 Email: mantegazza@ipmc.cnrs.fr

Education

■1998 Ph.D. in Physiology (highest grade) - University of Milan, Italy.

■1996 Professional habilitation - University of Milan, Italy.

■1992 Master (Laurea) degree in Biology (highest grade) - University of Milan, Italy.

■1987 Scientific High School Diploma - Como, Italy.

Main Scientific Interests

Ion channels and physiopathology of neuronal excitability; Epilepsy; Application of RNAi technologies; Peptidic toxins.

Training and professional experience

■2009 (November) - Inserm CR1/Group leader: IPMC, Nice-Sophia Antipolis, France.

■2008-2009 Inserm CR1/Group leader (Mission: Besta Neurological Institute, Milan, Italy)

■2001-2009 Besta Neurological Institute, Milan, Italy - Group leader. Ion channels and pathophysiology of neuronal excitability.

■1997-2001 Department of Pharmacology, University of Washington, Seattle, WA - Senior Fellow with William Catterall. Structure/function relationships and modulation of sodium channels.

■1994-1997 Besta Neurological Institute, Milan, Italy - Ph.D. Student - With Giuliano Avanzini and Enzo Wanke. Firing patterns of cortical neurons and mechanism of action of antiepileptic drugs.

■1993-1994 Dept. of Experimental Biomedical Sciences, University of Padova, Italy - Fellow with Daniela Pietrobon and Jürgen Hescheler (Institute of Pharmacology - Freie University of Berlin, Germany). Single channel analysis of modulation of calcium channels in pituitary cells.

■1991-1992 Dept. General Physiology and Biochemistry - University of Milan, Italy - Undergraduate Student with Enzo Wanke. Physiology and pharmacology of calcium channels and cholinergic receptors in sensory neurons.

Honors and awards

Human Frontier Science Program Long Term Fellowship, 1998−2000.

Atip/Avenir start-up grant (France), 2008-2012.

Editorial board of Frontiers in Pharmacology (Ion Channels and Channelopathies) (2010-)

Referee for Annals of Neurology, Brain, Brain Research, Brain Research Bulletin, British Journal Pharmacology, Clinical Evidence, Epilepsia, Epilepsy Research, Expert Review of Neurotherapeutics, FEBS Letters, Human Molecular Genetics, Human Mutation, Journal of Medical Genetics, Journal of Membrane Biology, Journal of Neurophysiology, Journal of Neuroscience, Journal of Physiology, Marine Drugs, Molecular and Cellular Neurosciences, Neurobiology of Disease, Neurogenetics, Neurological Sciences, Neuropharmacology, Neuroscience Letters, Pflugers Archiv-European Journal of Physiology.

Research proposal reviewer for Epilepsy Research Foundation (UK), Agence Nationale de la Recherche (ANR, France), Fondation pour la Recherche Medicale (FRM, France), University Paris V-Descartes, Emergence Program- City of Paris (France), Neurological Foundation of New Zealand.

Supervising

■2002-present. Four Ph.D. students: Physiology (University of Milano); Life Sciences (University of Nice – Sophia Antipolis). Eight undergraduate/master students: Biotechnology and Biology Degrees, University of Milano and Milano-Bicocca University; Life and Health Sciences (University of Nice – Sophia Antipolis).

Scientific summary

My main research interests are targeted to the study of ion channels and their influence on the pathophysiology of neuronal functions, and to the development of innovative molecular strategies for the treatment of pathological cellular excitability. We are interested in how ion channels shape neuronal physiological functions, in how their modifications cause pathologies and in how they are modulated or blocked by drugs and toxins. In particular, we are studying the different physiological functions of neuronal voltage gated Na+ channels in different neuronal subtypes and the functional effects of their genetic mutations, using animal models and experimental approaches that reproduce pathophysiological conditions. Furthermore, we are interested in the mechanism of trafficking and folding of ion channels, because we have found epileptogenic mutations that cause trafficking/folding defects and we are studying novel approaches to rescue the mutant channels. We are also developing new methods for modulating neuronal excitability by gene delivery and RNA interference, in order to better understand the mechanisms of generation of neuronal network activity and to find novel approaches for limiting pathological excitability.
 
Techniques.
Patch-clamp recordings (transfected cell lines, transfected neurons, dissociated neurons and brain slices). Classical microelectrode recordings and Field potential recordings (brain slices). Molecular biology (cloning, mutagenesis, qPCR). Western blot, CoIP. Confocal and quantitative microscopy. Viral transduction and RNA interference. Cell cultures. Animal models
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