1988-1989 Postdoctoral fellow - Prof A. L. Sonenshein, Mentor – Department of Molecular Biology – Tufts University, School of Medicine (Boston, USA)
1987 Ph.D (Doctorat d'état ès Sciences in Microbiology) – Université Paris VII
1982 Ph.D (Thèse de Doctorat de 3ème cycle in Biochemistry) - Université Paris VII
2016- present Co-Director of the team Barriers and Pathogens, Institut Cochin, INSERM U1016-CNRS UMR8104-University Paris Descartes
2011-present Research Director class II, CNRS, Institut Cochin, team Barriers and Pathogens
2002-2010 Research Director class II, CNRS, Insrtitut Pasteur, Team Toxines Pathogénie Bactériennes
1990-2002 Assistant Professor, CNRS, Insrtitut Pasteur, Team Toxines Pathogénie Bactériennes
1988-1989 Research Assistant, CNRS, Tufts Medical School, Boston MA
1985-1987 Research Assistant, CNRS, Institut Pasteur, Team Biochimie Microbienne
1984-1985 Research Assistant, Institut Pasteur, Team Biochimie Microbienne
– Editor-in-Chief for Microbiology, 2010 - 2015
– Senior Editor for Microbiology, 2002 - 2007
- Editor for Microbiology, 1995 - 2002
– Invited Editor for Research in Microbiology, Special issue: Sporeformers (2010).
Member of thesis (15) and habilitation (8) committees
Practical courses in the General Microbiology Course of the Institut Pasteur
Tutoring under-graduate students for bibliographical seminars
Medical sciences training, Faculty of Medicine, University Paris-Descartes Lecture: Bacteral toxins
- 4th European Spores Conference (2010, Cortona, Italy)
- Bacillus-ACT 2009 (Santa Fe, USA)
- Bacillus-ACT 2007 (Oslo, Norway)
- Bacillus-ACT 2005 (Santa Fe, USA)
- Bacillus-ACT 2003 (Nice, France)
2011- Host-pathogen interaction: Streptococci, GAS and GBS
Molecular mechanisms involved in the physiopathology of these pathogens:
- Bacterial virulence factors, wih a focus on surface exposed proteins.
- Interaction of these proteins with their with eucaryotic ligands and consequences on the cell.
Molecular epidemiology of GAS and GBS; evolution of the clinical isolates
1990-2011 Host-pathogen interaction: Surface components and regualtion of virulence factor synthesis in Bacillus anthracis
Regulation of synthesis of virulence and persistence factors:
Expression of toxin, capsule and S-layer genes are controlled in vitro by a common regulator, AtxA.
Link between expression of virulence factors and metabolic state of the bacterium. A metabolic regulator activates the expression of the toxin genes and of the iron metabolism operons
The capsule, a major virulence factor, is synthesized by a four protein complex and covalently anchored to the peptidoglycan by a transpeptidase
SLH harboring proteins require pyruvylation of the peptidoglycan-associated polysaccharide to be anchored.
The gamma phage receptor has been characterized; it is covalently anchored to the peptidoglycan
Anchoring domains of surface components efficiently present heterologous antigens
French B. anthracis strains belong to two dissimilar genetic clusters, geological strain patterns were observed.
On genetic basis, B. anthracis is a B. cereus. Regulator activities yield differential virulence factor syntheses.
1988-1989 Bacterial metabolism: sporulation initiation and catabolic repression in Bacillus subtilis
Repression of sporulation initiation by glucose and catabolic repression of genes encoding Krebs cycle enzymes are independent. Control by catabolite repression and control by components of nutrient broth act by different mechanisms.
1980-1987 Bacterial metabolism: sucrose metabolism in Bacillus subtilis
Bacillus subtilis imports and hydrolyzes sucrose and secretes an enzyme that degrades sucrose and polymerizes fructose into levans. Sucrose is imported via a PTS. B. subtilis enzyme IISuc promotes sucrose import when expressed in E. coli. The expression of the sucrose operons is subject to multiple regulations, that have been characterized at the molecular level.