Date of Birth: 08/02/1954 (57 years old);
Research Director at Inserm
Deputy General Director of Centre de Biophysique Moléculaire, CNRS UPR4301; Rue Charles Sadron 45071 Orléans cedex 2, France
E-mail : email@example.com Tel: 33 (0)2 38 25 55 65
1987 Doctor es Sciences (PhD equivalent), University of Orléans. Thesis title: Drug and immunomodulator targeting using monoclonal antibodies and glycoconjugates.
Research Director Inserm at CNRS UPR4301, Orléans, France, 1994-present
Research Assistant Inserm at CNRS UPR4301, Orléans, France, 1985-1993
Deputy General Director of Centre de Biophysique Moléculaire, CNRS UPR4301, 2008- 2012
Scientific team leader of “Gene transfer by synthetic vectors” (1999 - present) (15 members); Principal investigator of 32 academic grants.
h-index: 31; Total citations: 3100; Peer-reviewed publications: 80; Reviews: 10; book Chapters 14 ; Patents : 8; ; Invited conferences: 9
SUPERVISOR of 15 PhD students and 10 PostDoc. REPORTS OF PhD DEFENSES: 14; Reports of Habilitation to direct research (HDR): 2
MAJOR COMMITTEE ASSIGNMENTS.
- INSERM: 2008-2012; Elected Member at Scientific committee #7; CNRS: 2006 -2008: Member at the Laboratory and Scientific Committees of UPR4301.
- French Fundation for Myopathies Association Française contre les Myopathes (AFM). 2010-2014 Member at Scientific Council of the “Gene and-or Cell therapy of rare disorders” committee; Member at the Committee for General Overview of the AFM Strategic and Therapeutic Development (COSET) (2010-2013):
- National Ligue of Cancer (Ligue Nationale contre le cancer): 2008-2011; Member at the Committee for Project Evaluation.
- INCa (National Institute of cancer): 2006 ; Member of the Committee for project evaluation : Projets libres et réseaux structurants en Biologie du cancer dans la commission « Technologies innovantes en imagerie et radiothérapie »
- French Medical Research Council: 2009; Member at the Steering Committee of the call « Les recherches sur les cellules iPS comme modèles physiopathologiques ou outils de criblage de molécules thérapeutiques.
- ANR: 2009; Member at the Evaluation Committee of ANR EmergenceBio.
- University of Nice: 2009; Member at the Committee of the Recruitment Selection for Professor # 32.
- University of Orléans:
2009: President at the Committee of the Recruitment Selection for Assistant Professor # 64 -65.
2009: Membre du Comité scientifique disciplinaire
2006-2008: Member of Selection Committee # 64-65.
- AERES: 2008-2011: Member at 4 Evaluation Committees.
Member of Editorial Board of Journal of Gene medicine (2003-present); Human Gene Therapy (2009-present); Bioconjugate Chemistry (1998-2002).
MEMBER OF THE BOARD of the French Society of Cell and Gene Therapy (2008-2012 ).
My research activities concern the vectorisation of nucleic acids by non-viral delivery systems allowing the transfection of target cells. I have an outstanding expertise in nucleic acids vectorisation based on the use histidine-rich cationic polymers and cationic lipids to form DNA-nanoparticles. The major novelties concerning vectors for transfection are i) Histidinylated l-polyethyleneimine, ii) lipophosphoramidates containing imidazolium and histidine and iii) the design of an optimized DNA sequence (3NF) allowing NFκB-mediated nuclear import of plasmid DNA. These innovations represent a major advance in the field. By using those vectors, we have obtained very promising results for two in vivo applications: i) cancer vaccination by transfer of mRNA encoding tumor antigen and ii) Achilles tendon healing by gene transfer. Lastly, a novel method using ultrasounds and gas microbubbles so called sonoporation has been developed for the delivery of drugs and genes. A novel project was started to design undamaged and damaged DNA substrates capable to reach the cell nucleus and to act as a decoy oligonucleotide to define cellular mechanisms of MisMatch Repair system signaling. During this period, the team has acquired a strong expertise in intracellular trafficking investigations of DNA-nanoparticles by cell imaging. For the first time, we have visualized the nuclear import of a plasmid DNA and quantified the number copies in the nucleus of transfected cells.