1996- PhD in immunology
1997-2003 Post-doctoral position in Pr Colin Watts Laboratory (Dundee, Scotland)
2004-2010 AVENIR team, CR1 position and PI in Institut Curie
2011- Investigator in Pr Van Endert Laboratory U1013 "Tolérance immunitaire et présentation antigènique"
The innate immune system provides the first barrier against pathogens. At the steady state, intracellular TLRs (TLR3, 7, 8 and 9) are retained in the endoplasmic reticulum together with a chaperone molecule, UNC93B1. Upon stimulation, they relocate to the endo/lysosomal compartment, thus allowing binding to their ligands and the recruitment of their adaptor molecules, MyD88 or TRIF. It has been shown that mouse TLR9 requires proteolytic cleavage in the endosomes to be functional. We are investigating which proteases regulate endosomal TLRs signaling and the role of these TLRs and chaperone molecules in the MHC class I antigen cross presentation pathway in dendritic cells. Our results indicate that AEP is a unique protease processing TLR7 and TLR9 in dendritic cells opening new therapeutical possibilities for the treatment of inflammatory and cancer diseases where TLR7 and 9 pathways are dysregulated.