Scientific topics
Keywords
Thomas Lefebvre PhD in Neurosciences
Course and current status
2010 - 2014 : SynAging (Nancy, France)
Since 2014 : Actelion (Allschwil, Suisse)
Scientific summary
Scientist PhD drug discovery-oriented, specialized in GPCRs and ion channels with experience in Neurosciences and Immunology.
Topics of studies: screening, GPCRs (desorphanization, signaling and dimerization), ion channels (pharmacology and physiology), nervous system diseases and animal behavior.
Skills: Project Management - Assay Development - Preclinical Pharmacology
- Drug discovery
- Cell-based assays: GPCR signaling, cytotoxicity assays, FACS, siRNA
- Cell culture: tissue slices, microexplants, primary cells, recombinant mammalian cells
- Molecular biology: RNA extraction, Q-RT-PCR, PCR, subcloning, gel agarose electrophoresis
- Stereotactic surgery: injection, lesioning and cannulation
- Small molecules screening using phenotypic screen
- Histology: immunofluorescence and image analysis by confocal microscopy
- Patch-clamp electrophysiology: whole-cell recordings, neuronal activity
