Sophie Peron Ph.D Immunology
Course and current status
Current position
Since November 2013 : Tenured term position at Unit CNRS UMR 7276, Limoges as INSERM investigator.
Education
January 2022 : Habilitation to supervise research (HDR), Limoges University, Immunology.
September 2016 : Education and training course in laboratory animal experimentation, function B. Ecole Nationale Vétérinaire de Toulouse (ENVT).
August 2004 - Sept. 2008 : PhD in Immunology at University of Paris VII and Inserm U768, Necker Hospital. Thesis : “Molecular characterization of inherited immunoglobulin class switch recombination deficiencies”. Director : Dr. Anne Durandy. Thesis defense : 15th of September 2008
Sept. 2003 -July 2004 : M.Sc Immunology at University of Paris VI. Advanced Immunology course of Pasteur Institute (Paris).
Sept. 1999 - July 2003 : Post-graduate degrees in Biochemistry, Immunology and Microbial Biochemistry at Poitiers University.
Fellowships
August 2007 – Sept. 2008 : Fellowship from French Foundation for Medical Research (FRM).
August 2004 - Sept. 2007 : Fellowship from French Ministry for Higher Education and Research.
Previous position
2009 – 2013 : Post-doctoral Fellow at Unit CNRS UMR 7276, Limoges.
Teaching activity
- Master Molecular oncology and biotherapy, Science faculty, Limoges University : lectures on mechanisms and regulation of Immunoglobulin gene recombinations (2017- present).
- Master Genetic, Physiology and Biotechnology, Science faculty, Limoges University & University of Clermont-Ferrand: lectures on mechanisms and regulation of Immunoglobulin gene recombinations (2013-2016).
- Immunology for biological analysis technician: Immunology lectures and immune-analysis technique praticals (2012-2013).
- Immunology for students from Faculty of Pharmacy: Innate Immunology lectures, Cellular Biology techniques (2012-2013).
- Master Infection and immunity, Science faculty Limoges University: tutorial classes (2010-2011).
Selected oral presentations
Name of presenting author is underlined
IgH Locus Scuicide Recombination (LSR) in Chronic Lymphocytic Leukemia (CLL) : prognosis indicator? Israa AL JAMAL, Nathalie GACHARD, Samar AL HAMAOUI, Nehman MAKDISSY, Jean FEUILLARD, Sophie PERON. 17émes Journées du Cancéropôle GSO, November 2021, Carcassonne, France.
Recombinaison suicide du locus IgH dans la LLC : indicateur de pronostic ? Israa AL JAMAL, Nathalie GACHARD, David RIZZO, Jean FEUILLARD, Sophie PERON. Annual congrees of the Société Française d’Hématologie, September 2021, Paris, France.
Study of IgH Locus Suicide Recombination ‘LSR’ in human normal and pathological condtions (Chronic Lymphocytic leukemia). Israa Al Jamal, Nathalie Gachard, Nehman Al Makdissy, Samar Al Hamaoui, Jean Feuillard et Sophie Peron. International 3rd European B Cell Forum, July 2021.
Does a balance exist between class switch recombination and locus suicide recombination in B-cells and whether it is influenced by the DNA repair pathways? Péron S, H. Boutouil, I. Al Jamal, F. Boyer, J. Moreau, N. Gachard, M. Cogné, J. Feuillard. "Mechanisms and consequences of Chromosomal Translocations in Cancer » meeting, May 2019, Paris, France.
Study of the transcription of the IgH locus 3’ regulatory region (3'RR) during the maturation of B lymphocytes. Péron S, Cook-Moreau J, Cogné M. « Génome, structure et fonctions » workshop, Cancéropôle GSO, April 2013, Toulouse, France.
Immunoglobulin heavy chain Locus Suicide Recombination in mature B cells. Péron S, Laffleur B, Denis-Lagache N, Cook-Moreau J, Tinguely A, Delpy L, Denizot Y, Pinaud E, Cogné M. Keystone Symposia « Mutations, Malignancy and Memory-Antibodies and immunity”, March 2012, Boston, United States.
New autosomal recessive Forms of HIGM caused by an intrinsic B cell defect. Peron S, Forveille M, Fischer A, Durandy A.16th European Congress of Immunology , September 2006, Paris, France.
Available on demand
CSReport software (Boyer et al., J Immunol. 2017) allows the analysis of high-throughput sequencing data of IgH locus recombination junction PCR amplifications. CSReport requires Python3 and Jupyter environments (preferably from an Anaconda distribution) with updated Biopython package. Raw sequencing data should be single-end reads (in multi-fasta or fastq formats). CSReport works with a custom reference (derived from NCBI sources) of a constant IgH locus sequence and annotations (humans or mice). The reference should be chosen according to the model organism. The CSReport notebook file (ZIP archive, ) including the reference files is available on request by email.
Awards
-Peron S, Familiades J, Brousset P, Delabesse E, Broccardo C, Delpy L, Cogné M. Analysis of the function on 3’RR of PAX5 isoforms and PAX5-P80R mutant. 6èmes journées du Cancéropôle GS0, Toulouse, France (2010). Selected poster for Fondamental Research Award.
-Peron S, Forveille M, Fischer A, Durandy A. Analysis of DNA double strand break repair in immunoglobulin class switch recombination deficiency. Normal and tumoral B-cell congress, Ile d’Oléron, France (2005). Poster Award.
National membership
Member of the French Society of Hematology
Member of the French Society of Immunology
-h-index (Scopus) : 13 (February 2022). Number of publications : 19, Number of citations : 701.
Scientific summary
My research work focuses on B lymphocytes (LB) and mainly on the exploration of recombination of the IgH locus of immunoglobulins (Ig). This theme required me to acquire, in addition to knowledge in immunology, knowledge in the fields of the regulation of gene accessibility, DNA mutation and DNA repair. My doctoral internship was carried out in the team of Doctor Anne Durandy in the laboratory directed by Professor Alain Fischer at the Necker hospital in Paris. During this work, we have identified primary immune deficiencies due to Ig isotype switching defect due to alterations in DNA repair (Peron JEM 2007, Peron JEM 2008). My post-doctorate and the beginning of my career as a permanent researcher at INSERM were carried out at the CRIBL laboratory "Control of the B immune response and lymphoproliferation" in Limoges within the team led by Professor Michel Cogné between 2009 and 2018, “Molecular genetics of the B cell and immunoproliferative syndromes”, and from December 2018, in the “Molecular mechanisms of lymphomagenesis” team led by Professor Jean Feuillard. Following my doctoral internship centered in human physiology and pathology, I wanted to approach the research work that uses the mouse model in order to learn the design of this tool and master its use and manipulation. This opportunity was given to me during my post-doctorat by developing a mouse model expressing a mutant of the PAX5 transcription factor, PAX5-P80R, specifically in the B lymphoid lineage. My work on the study of the he activity of the PAX5 isoforms in the LB allowed the detection for the first time of the suicide recombination of the IgH locus (LSR) (Peron Science 2012) which I continued to study in mice. Finally, motivated by the desire to work on human cells, considering the singularities inherent in human cells, and particularly on cancers, I chose to join Professor Jean Feuillard's team at the end of 2018. This is how I obviously joined the working group on Chronic Lymphoid Leukemia (CLL): the direction I wanted to give to my research theme and my skills in the biological fields concerned by genetic rearrangements crossed the questions from the group already formed and already recognized for its expertise on non-Hodgkin's B lymphomas in general and on CLL in particular. During all of my research work within the CRIBL laboratory, I had the opportunity to supervise student trainees (master students and Ph.D students).
Research
Chronic lymphocytic leukemia (CLL) is a malignant hemopathy caused by the monoclonal proliferation of mature B-cells/B Lymphocytes. According to the report « Les cancers en France » (Les cancers en France, Les Données/ Epidémiologie. INCa,2017), in 2012 in France there were 35,000 new cases of malignant hemopathies per year half of which are lymphoid hemopathies which include cancers of the B lymphocyte lineage. CLL is a non-Hodgkin lymphoma and the most frequent leukemia in adults and older adults are most commonly affected. CLL results in circulatory and lymphnode lymphocytosis (increased lymphocyte count) which deregulates the immune response, increasing the risk of infections and cancers. The oncogenic processes of tumoral transformation in CLL remain unclear.
Using a tool developed by our team (Boyer et al., J Immunol. 2017) allowing the analysis of genetic recombination of the immunoglobulin heavy chain locus from high throughput sequencing data, using chromatin immunoprecipitation experiments and developing functional tests, we are analyzing DNA repair in tumor cells of patients suffering from CLL.
We aim to understand the biological consequences of DNA repair mechanism deregulation in tumoral transformation in CLL.
Associated members:
Kenza guiyedi (PhD student 2021- )
Milène Parquet (PhD student 2021- )
Israa Al Jamal (PhD student 2018- )
Hend Boutouil (PhD student, 2015-2018)
