Right after graduating from engineering school, I worked two years in San Francisco, California for two start-ups. The first one was a CRO of three employees and the second one was a 20 employees biochemistry lab, developping kits for protein characterization.
I came back to France in Nantes and worked 2 years for a recombinant protein facility in the CRCINA. There I was in charge of protein production and purification for many public research teams but also developped a technique for the screening of T-cells epitope with HLA-E/peptide complexes.
I now work for the research team of Cécile Duplàa in Pessac where I specialized myself in histology, cellular biology and molecular biology.
Cellular Biology :
- Culture and transcient transfections of cancerous but also primary cells
- Culture of hybridomes for protein production
- Large variety of function tests (cell migration, polarity under different flows, permeability, proliferation, transcytocis, sprouting, spreading, focal adhesion...)
Protein production and purification :
- FPLC/HPLC : BioRad, Waters and Cytiva (ex General Electric) systems
- production in eukaryotic and prokaryotic cells
- characterization with Nanotemper technology
Molecular biology basic knowledge (Western blots, ELISA, PCR, RT-qPCR...)
Confocal Mycroscopy
Histology (Microtome, Cryotome, Vibratome, Transparization...)
Softwares : Imaris, Fiji, GraphPad, Office, SnapGene, Zen