Dongdong Li
  • E-mail :[email]
  • Phone : +33 1 44 27 33 57
  • Location : Paris, France
Last update 2022-10-04 19:30:32.382

Dongdong Li PhD in Biomedical Engineering

Course and current status

2010 -               Researcher@Inserm (National Institute of Health and Medical Research/L'Institut national de la santé et de la recherche médicale, France), Paris

2005 - 2010      Post-doc, Neurophysiology and new microscopies laboratory, INSERM, Université Paris Descartes, Paris

2000 - 2005      PhD Biomedical engineering, Huazhong univ sci&tech, Wuhan

1993 - 1997      B.E. Electrical automation, Inst. tech, Tianjin

Scientific summary

Neuron/glia signaling in health and disease

The mammalian brain consists of comparable number of neurons and glial cells. An important challenge now in neuroscience is to understand how neurons and glial cells co-operate to regulate the function and health of the nervous system.

We currently study the signaling baiss of neuron-glia communication, ranging from second messenger dynamics (e.g., Ca2+, cAMP), transmitter release, to vesicle/receptor trafficking. Not only will it provide integrative knowledge on neurophysiology, but also help to identify dysregulations in neuron/glia actions in neurological diseases. A multidisciplinary approach is being employed, including (but not limited to) optical imaging, genetic engineering, pharmaco-/optogenetics, and electrophysiology.

Publications (https://orcid.org/0000-0002-6731-4771):

Hypothalamic astrocytes control systemic glucose metabolism and energy balance. Herrera Moro Chao D, Kirchner MK, Pham C, Foppen E, Denis RGP, Castel J, Morel C, Montalban E, Hassouna R, Bui LC, Renault J, Mouffle C, Garcias-Caceres C, Tschöp MH, Li D, Martin C, Stern JE, Luquet S. Cell Metabolism. 2022 Oct 05; https://doi.org/10.1016/j.cmet.2022.09.002. 

Dyclonine inhibition of TRPV3: From functional discovery to structural insight. Wang P, Mo X, Li D, Yao J. Cell Calcium. 2022 Jun 22;105:102617. doi: 10.1016/j.ceca.2022.102617. 

Tyrosine phosphorylation tunes chemical and thermal sensitivity of TRPV2 ion channel. Mo X, Pang P, Wang Y, Jiang D, Zhang M, Li Y, Wang P, Geng Q, Xie C, Du HN, Zhong B, Li D, Yao J. eLife. 2022 Jun 10;11:e78301. doi: 10.7554/eLife.78301. 

Identification of an arthropod molecular target for plant derived natural repellents. Tian Q, Wang P, Xie C, Pang P, Zhang Y, Gao Y, Cao Z, Wu Y, Li W, Zhu MX, Li .D, Yao J. PNAS. 2022. May 3;119(18):e2118152119. doi: 10.1073/pnas.2118152119. Epub 2022 Apr 22. 

The industrial solvent 1,4-dioxane causes hyperalgesia by targeting capsaicin receptor TRPV1. Mo X, Liu Q, Gao L, Xie C, Wei X, Pang P, Tian Q, Gao Y, Zhang Y, Wang Y, Xiong T, Zhong B, Li D, Yao J. BMC Biol. 2022 Jan 7;20(1):10. doi: 10.1186/s12915-021-01211-0. 

Lactate is an energy substrate for rodent cortical neurons and enhances their firing activity. Karagiannis A, Gallopin T, Lacroix A, Plaisier F, Piquet J, Geoffroy H, Hepp R, Naudé J, Le Gac B, Egger R, Lambolez B, Li D, Rossier J, Staiger JF, Imamura H, Seino S, Roeper J. Cauli B. eLife. 2021 Nov 12;10:e71424.

Therapeutic inhibition of keratinocyte TRPV3 sensory channel by local anesthetic dyclonine. Liu Q, Wang J, Wei X, Hu J, Ping C, Gao Y, Xie C, Wang P, Cao P, Cao Z, Yu Y, Li D, Yao J. eLife. 2021 April;10:e68128. doi: 10.7554/eLife.68128.  

Astrocytes respond to a neurotoxic amyloid fragment with state-dependent Ca2+ alteration and multiphasic transmitter release. Pham C, Hérault K, Oheim M, Maldera S, Vialou V, Cauli B, Li D. Acta Neuropathol Commun. 2021 March 9: 44. https://doi.org/10.1186/s40478-021-01146-1.  

Flotillin-1 interacts with and sustains the surface levels of TRPV2 channel. FHu J, Gao Y, Huang Q, Wang Y, Mo X, Wang P, Zhang Y, Xie C, Li D, Yao J. ront Cell Dev Biol. 2021 Feb 9;9:634160. doi: 10.3389/fcell.2021.634160.   

Site-specific contacts enable distinct modes of TRPV1 regulation by the potassium channel Kvb1 subunit. Wang Y, Mo X, Ping C, Huang Q, Zhang H, Xie C, Zhong B, D Li, J Yao. J Biol Chem. 2020 october. doi: 10.1074/jbc.RA120.015605. 

Mapping astrocyte activity domains by light sheet imaging and spatio-temporal correlation screening. Pham C, Herrera Moro D, Mouffle C, Didienne S, Hepp R, Pfrieger FW, Mangin JM, Legendre P, Martin C, Luquet S, Cauli B, Li D. NeuroImage. 2020 June, https://doi.org/10.1016/j.neuroimage.2020.117069. 

Recovery from tachyphylaxis of TRPV1 coincides with recycling to the surface membrane. Tian Q, Hu J, Xie C, Mei K, Pham C, Mo X, Hepp R, Soares S, Nothias F, Wang Y, Liu Q, Cai F, Zhong B, Li D, Yao J. doi: 10.1073/pnas.1819635116. PNAS. 2019 Feb 25; 

Concerted interneuron activity in the cerebellar molecular layer during rhythmic oromotor behaviors.Astorga G, Li D, Therreau L, Kassa M, Marty A&Llano I. J Neurosci    

Expression and cellular function of vSNARE proteins in brain astrocytes. Ropert N, Jalil A, Li D. Neuroscience. 2016 May 26;323:76-83. 

Astrocyte VAMP3 vesicles undergo Ca2+ -independent cycling and modulate glutamate transporter trafficking. Li D, Hérault K, Zylbersztejn K, Lauterbach MA, Guillon M, Oheim M, Ropert N. J Physiol. 2015 Jul 1;593(13):2807-32. 

New tools for investigating astrocyte-to-neuron communication. Li D, Agulhon C, Schmidt E, Oheim M, Ropert N. Front Cell Neurosci. 2013 Oct 29;7:193.

Lack of evidence for vesicular glutamate transporter expression in mouse astrocytes. Li D, Hérault K, Silm K, Evrard A, Wojcik S, Oheim M, Herzog E, Ropert N. J Neurosci. 2013 Mar 6;33(10):4434-55. 

 Calcium rubies: a family of red-emitting functionalizable indicators suitable for two-photon Ca2+ imaging. Collot M, Loukou C, Yakovlev AV, Wilms CD, Li D, et al. J Am Chem Soc. 2012 Sep 12;134(36):14923-31. 

Optogenetic activation of LiGluR-expressing astrocytes evokes anion channel-mediated glutamate release. Li D, Hérault K, Isacoff EY, Oheim M, Ropert N. J Physiol. 2012 Feb 15;590(4):855-73. 

FM dyes enter via a store-operated calcium channel and modify calcium signaling of cultured astrocytes.Li D, Hérault K, Oheim M, Ropert N.  PNAS. 2009 Dec 22;106(51):21960-5. 

Measuring mitochondrial and cytoplasmic Ca2+ in EGFP expressing cells with a low-affinity CALCIUM RUBY and its dextran conjugate. Luccardini C, Yakovlev A, Pasche M, Gaillard S, Li D, et al. Cell Calcium 2008. 45:275-283.   

Lysosomes are the major vesicular compartment undergoing Ca2+-regulated exocytosis from astrocytes.  Li D, Ropert N, Koulakoff A, Giaume C, Oheim M. J Neurosci. 2008 Jul 23;28(30):7648-58.

Systematic colocalization errors between acridine orange and EGFP in astrocyte vesicular organelles. Nadrigny F, Li D, Kemnitz K, Ropert N, Koulakoff A, Rudolph S, Vitali M, Giaume C, Kirchhoff F, Oheim M. Biophys J. 2007 Aug 1;93(3):969-80. 

In Imaging Cellular and Molecular Biological Function. - F. Frischknecht, J. Pawley and S. Shorte, eds. Springer, Berlin Heidelberg. 2006. pp.117–156. Oheim M and Li D. Quantitative Co-localisation Imaging: Concepts, Measurements and Pitfalls. 

Differential properties of GTP- and Ca2+-stimulated exocytosis from large dense core vesicles. Bai L, Zhu D, Zhou K, Zhou W, Li D, et al. Traffic 2006 7:416-428.  

 Dynamic difference between GLUT4 storage vesicles and secretory vesicles revealed by evanescent-field imaging and single particle tracking.  Liang T, Li D, Xiong J, Zhu D, Qu A. Acta Biophysica Sinica 2005 21:261-268.

Rapid biogenesis and sensitization of secretory lysosomes in NK cells mediated by target-cell recognition. Liu D, Xu L, Yang F, Li D, Gong F, and Xu T. PNAS 2005 102:123-127. 

Neural Interface and Control –IEEE 2005pp.225-229. Li D, Liang T, and Qu A. Quantitative analysis of diffraction-limited three-dimensional single secretory vesicle tracking in live neuroendocrine cells. 

Conf Proc IEEE Eng Med Biol Soc 2005. 7:7529–7532. Xiong J, Li D, Zhu D, and Qu A. Three-Dimensional tracking of single granules in living PC-12 cells employing TIRFM and WFFM. 

 Three-dimensional tracking of single secretory granules in live PC12 cells.  Li D, Xiong J, Qu A, and Xu T. Biophys J 2004 87:1991-2001.

Dynamic tracking and mobility analysis of single GLUT4 storage vesicle in live 3T3-L1 cells. Li C, Bai L, Li D, Xia S, and Xu T. Cell Res 2004 14:480-486. 

Image d’exemple