CV Science

daniele praseuth PhD Biochemistry

Course and current status

1985: PhD thesis Development and characterisation of hydrosoluble and photoactivatable  porphyrins (Laboratoire de Chimie de Coordination Bioorganique, Pr A. Gaudemer LA 255, and Laboratoire de Biologie Moléculaire Végetale, LA40, Pr F. Quétier).Université Paris-Sud. 91405 Orsay, France

1986: Post-doctoral stage 1: Laboratoire de Biophysique directed by Pr C. Hélène, Museum National d'Histoire Naturelle MNHN (financial support: Association pour la Recherche sur le Cancer). Development of photo-activatable molecules to target sepcific nucleic acids sequence.

1987-88: Post-doctoral stage 2:INSERM U 200 (Immunologie de l’allergie et de l’inflammation, Pr J. Benvéniste), Universite Paris-Sud, Clamart, France- Characterisation of some immunologic properties of Paf-acether on lymphocytes.

1989: Nomination Maitre de conference ( Laboratoire de biophysique du MNHN, Pr C. Hélène)

Scientific summary

Development of synthetic oligonucleotides as versatile tools to modulate gene expression

Targeted damage in specific nucleic sequences using activatable antisense or antigene oligonucleotides:

Publications: Praseuth et al. 1987, 1988a et b, 1990; LeDoan et al. 1987 et 1990; Birg et al., 1990; Noonberg et al. 1995; Hégarat et al, 2014

Modulating protein-nucleic acid interactions by triplex formation in a specific sequence context results in specific inhibition of transcription or damage repair. Optimizing antigene strategy to regulate transcription, looking for specific cellular proteins involved in triplex recognition:

Publications: Grigoriev et al., 1992; 1993a et b; Praseuth et al., 1993; Guieysse et al.,1995a et b, 1996, 1997, 2000; Hélène et al. 1997; Praseuth  et al. 1993, 1996, 1999; Guillonneau et al., 2004.

Generic and unbiased methods to purify and identify proteins specific for nucleic acids sequences and  structures (triplex, DNA ends, others):

Publications: Guillonneau et al, 2001a and b; Rusconi et al., 2002; Guipaud et al, 2006; Hégarat et al 2007 et 2008; Kanoush et al, 2011; Berthelot et al, 2016

Proteomic analysis applied to toxicology:  differential analyse applied to study biological effects of microcystine-LR exposure on liver from medaka fish(Collaboration with DR M. Edery, MNHN):

Publications: Mezhoud et al., 2008a, b et c,  2009; Malécot et al, 2009