CV Science

Vincent Gache CR1 INSERM, PhD in Cell Biology & Biochemestry

Course and current status

Chargé de Recherche, CR1 INSERM, Principal Investigator (PI).

Institut NeuroMyogène (INMG) Muscle Nuclear & Cytoskeleton Architecture Team (MNCA Team) CNRS UMR 5310 - INSERM U1217 - University of Lyon.

Project: Interplay between cytoskeleton network regulation, myonuclear domains establishment and muscle function during muscle development and in different forms of myopathies

- Postoctoral Research in cellular biology in Dr. Laurent Tiret Lab, école Nationale Vétérinaire d’Alfort, ENVA, Maison Alfort. France.

Project: HACD1 role in the regulation of myoblast fusion and skeletal muscle growth

- Postoctoral Research in cellular biology in Dr. Edgar Gomes team, Mix Research Unite INSERM-UPMC Paris 6- Myology institute UMRS 787, GROUPE MYOLOGIE. Team 3 (Head Director: Dr. David. SASSOON).

Project: Spatial distribution of nuclei in muscle fibers: a novel actor of muscular function?

- Postoctoral Research in biochemestry & cellular biology in Dr. Andrei Popov Team, Laboratory of cytoskeleton (Head Director: Dr D. JOB), INSERM (U366) DRDC/CEA, Grenoble.

Project: Exploration of the Xenopus microtubule-associated meiotic proteome and identification of novel spindle components. Production of monoclonal antibodies against microtubule-associated meiotic proteome.

- PhD research in cellular biology. Joseph Fourier University, Grenoble. PhD fellowship from the French Ministry of Research and Technology (MENRT). Laboratory of cytoskeleton (Director: Dr. Didier JOB, Supervisor: Dr. Odile Valiron), INSERM (U366) DRDC/CEA, Grenoble.

Thesis title: Identification of proteins binding the native tubulin dimer.

Scientific summary

I am currently leading a team (MNCA for Muscle Nuclear and Cytoskeleton Architecture team) in Neuromyogène Institute (INMG). We are highly focused on understanding mechanisms controlling cytoskeleton rearrangements, myonuclear domain establishment & maintenance during muscle differentiation and their implication in pathological contexts such as genetic disorders (Emery-Dreifuss Muscular Dystrophy, Centronuclear myopathies…) or physiological aging (Sarcopenia). In the normal course of muscle development or regeneration, nuclei are first found in the center of myotube before migrating to the periphery of the mature skeletal myofiber. It has long been hypothesized that nuclei are normally positioned to the periphery of the fiber to stay out of the way of the contractile machinery. In fact, nuclei mispositioned in the center of myofibers are a hallmark of a class of muscular diseases called centronuclear myopathies. There are growing supports for a direct connection between the regulation of nuclear positioning and normal function of the muscle fibers. However, the comprehensive molecular network involved in how myonuclei are positioned throughout myogenesis, as well as the functional impact of mispositioned myonuclei in skeletal muscle remain poorly characterized. The long-term goal of MNCA team is to understand how nuclei/organelles are positioned in developing muscle, and how that relates to diseases.