CV Science

Eduardo ANGLES-CANO M.D., Sc.D. Vascular Biology Haemostasis (fibrinolysis) and Thrombosis

Course and current status

Education (degrees, dates universities)

Bachelor Sciences, University of Mexico (1965)

Medical Doctor, University of Mexico  (1971)

Internal Medicine-Haematology, Nat Ins Med Sc Mx (1975)

Master Biological Sciences, University of Paris (1977)

Sciences Doctor, University of Paris (1980)

Career/Employement (employers, positions and dates)

Intern in Medicine, National Nutrition Institut, Mexico City                 1972 -1974

Chief Resident, National Nutrition Institut,                                        1974 -1976

Resident in Hematology, Hôpital Saint-Louis, Paris                             1976 -1978

Fellowship in Hemostasis, Hôpital Cochin-INSERM,  Paris                    1979 -1981

Full time investigator, INSERM, Paris                                                 1982 -1989

Director of Research 2nd Class, INSERM, Paris                                   1989-1995

Director of Research 1st Class, INSERM, Paris                                   1996-2012

Director of Research, Emeritus, INSERM, PARIS      2012-

Specialization (specify)

(1)       main field                   Biochemistry of Hemostasis and Fibrinolysis

(2)       other fields                 Vascular Biology

(3)       current research interests

                                               NETs and fibrinolysis. Sepsis-neutrophils-platelets-microvesicles-plasminogen-thrombus lysis in cardio and neurovascular pathology

Scientific summary

With my graduate students and co-workers we are pioneer in the study of fibrinolytic microvesicles (MVs) as functional messengers of plasmin generation. Since plasminogen activation is a property not only of vascular cells but also of other cell types including epithelial and inflammatory cells, our working hypothesis is that MVs derived from cells that express plasminogen activators may generate, disseminate and transfer plasmin proteolytic activity (Blood, 2007). For instance, besides endothelial cells and leucocytes, neurons are able to release microvesicles bearing plasmin and tPA activity (Biochem J 2010). We have found that endothelial cell- and leucocyte- derived microvesicles bear the machinery necessary for plasmin formation including plasminogen activators and its receptors: tPA for endothelial MVs and the uPA/uPAR system for leukocyte MVs (Haematologica 2013).  

Furthermore, we have discovered a new mechanism for plasmin formation that bypass the requirement for co-assembly of plasminogen and uPA on the same surface (Blood 2010). These heterotypic cell-to-cell (platelets/ monocytes; platelets/MPs), cell-to-matrix (leukocytes/fibrin), or MPs-to-matrix (MPs/fibrin) proteolytic cross-talk represents an alternative pathway for localized plasmin formation that may be relevant to processes implicating cell migration and MP dissemination, i. e. inflammation or angiogenesis. Finally, our data provide additional evidence for a novel role of MPs and platelets, as vectors that generate and propagate plasmin fibrinolytic and/or proteolytic activity, and could thereby constitute a pharmacologic tool.

Presently, at the Inserm reaserch unit UMR_S 1140 (Faculty of pharmaceutical and biological sciences, Paris) we are studying the fibrinolytic and microvesiculation properties of human bone marrow mesenchymal stem cells as a potential tool for cell therapy in ischaemic diseases.  We are also studying the role of microvesicles in fibrinolysis and NET transformation of PMN in ischaemic diseases.

Current fields of interest:

-        Fibrinolysis and NETs in sepsis and cardiovasular ischaemic disease (ANR ENDOPAROMP).

-        Microvesicles: development of an original method of detection using binuclear metalic coordination complexes (ANR COCERP)