CV Science

Hélène ARNOULD PhD in Cellular and Molecular Biology

Course and current status

• Professional profil

Passionate PhD graduate with extensive experience in cellular and molecular biology experiments on stem cells and neurons. Aiming to use my organizational and methodological skills to be rapidly autonomous and efficient in the laboratory. Open to dialog, I can work in a team and adapt to any situation. Possess a training in BSL2 and BSL3 laboratory. 

• Work experience

. 2016 - 2019 PhD of Molecular and Cellular Biology - UMR INSERM 1124 - Stem cells, signalling and prions (B. Schneider)

From physiological function of cellular prion protein to neurodegenerative diseases

. 2016 - 2019 Teaching in Biochemistry - Bachelor's tutorials and practical work - Université de Paris

. 2015 - 2016 Master year 1 and 2 internships - UMR INSERM 1124 - Stem cells, signalling and prions (B. Schneider)

Signalling pathways controlled by cellular prion protein involved in neuronal stem cells homeostasis

. 2014 Bachelor internship - UMR CNRS 7369 - Extracellular matrix and cellular dynamic (M.P. Courageaot)

Impact of exposition to matrikines on cell cycle

• Education

. 2016 - 2019 PhD of Molecular and Cellular Biology - Université de Paris

. 2014 - 2016 Master major Stem cells - Sorbonne University (Paris)

. 2011 - 2014 Bachelor of Biology, Biochemistry, Earth and Environment, major biology and physiology - first of promotion - Reims Champagne-Ardenne University (Reims)

Scientific summary

• Resume

My  PhD was focused on new roles of cellular prion protein (PrP^C) in neurons. In a first published article, I was associated to a work showing a role of PrP^C in cell adhesion via the PrP^C/MARCKSL1/NCAM1 pathway. My work also contributes to reveal for the first time a key role of PrP^C (mainly  known for its function in neurodegenerative diseases) in stem cell homeostasis through control of energetic metabolism. This work, identifying signalling pathways by which PrP^C controls stem cells orientation towards mitochondrial oxidative degradation of glucose, will soon be send to submission. I also worked on a third aspect of PrP^C: its deregulation in neurons causes neurodegeneration not only in prion- and Alzheimer's diseases, but also in Parkinson's disease and Amyotrophic Lateral Sclerosis via the PDK1/TACE/TNFR1 pathway.

• Scientific communications

. Publications

> see the link "PubMed publication"

. Congress

> 10^th scientific day of Reseau francophone de métabolomique et fluxomique (June 2016, Montpellier, France)

> 7^th Iberian Congress on Prions 2018 (October 2018, Laguardia, Spain)

> Prion 2019 (May 2019, Edmonton, Canada) - 3^rd price in poster presentation

• Skills

. Scientific techniques: stem cells and neurons culture (BSL1 and BSL2); transfection; qRT-PCR; western blot; immunofluorescence; metabolic dosages (pyruvate, ATP), seahorse experiment (mitochondrial respiration)

. Project management: write a project; plan experiments; analyse results; bibliographic monitoring; orders and materials managements

. Computer skills: Office suite (word, excel, power point); internet (fully proficient); statistics (prism); pictures treatment (ImageJ); bibliographical database (Zotero); database analysis (Ingenuity)

. Languages: French (native); English (fluent, B2-C1); American Sign Language (ASL, learning)

. Safety and Ethic: training in charge of confined laboratory (BSL2/BSL3); training rescuer and first aid (09/2019); training in scientific ethic (01/2018)